The effects and mechanism of the pseudomonas aeruginosa mannose sensitive haemagglutination vaccine on the cell cycle of hepatocellular carcinoma

Abstract

Objective To investigate the effects and mechanism of the pseudomonas aeruginosa mannose sensitive haemagglutination vaccine (PA-MSHA) on the cell cycle of hepatocellular carcinoma (HCC).Methods MHCC97L and HepG2 cells were cultured and treated with different doses of PAMSHA and mannose.MTT and flow cytometry were used to measure cell proliferation and cell cycles,respectively.The expressions of cell cycle related proteins Cyclin D1,CDK2,PCNA,p21,and p27 were evaluated by Western blot.Results Compared with the control group,the proliferation of HCC Cells in the PA-MSHA treated group was significantly inhibited in a time and dose dependant manner (P＜0.05).PA-MSHA significantly arrested HCC cells in the G0-G1 and G2-M phase of the cell cycle,thereby decreasing the proportion of cells in the S phase (P＜0.05).Western blot analysis revealed that PA-MSHA significantly inhibited the expressions of Cyclin D1,CDK2,PCNA,and significantly promoted the expression of p21 and p27.Preincubation of PA-MSHA with mannose significantly inhibited the effects of PA-MSHA on proliferation and cell cycle (P＜0.05).Conclusion By regulating the expression of cell cycle related proteins,PA-MSHA significantly induced HCC cell cycle arrest and inhibited cell proliferation.These effects were mediated through PA-MSHA recognizing and combining to the mannose of HCC cells. Key words: Hepatocellular carcinoma; Pseudomonas aeruginosa vaccine; Mannose; Proliferation