Abstract
The effectiveness of conservative treatment in patients with knee joint arthritis can be enhanced through the use of the linked pharmacological and physical factors. The possibility of comprehensive use of low-intensity laser radiation (LILR) chondroprotectors and nonsteroidal anti-inflammatory drugs in patients with early knee joint arthritis- depending on the consistency of their application is not well studied. Objective: to study in experimental animals with induced knee joint arthritis effectiveness of different integrated schemes of conservative treatment. Methods: we conducted experimental studyin 40 white rats aged 6 months. Knee joint arthritis stage I modeled by triple dexamethasone injections (7 mg/kg/m)performed once a week. The rats were divided into six groups depending on the treatment, which included 10 daily sessions of at la-ser therapy (a dose of 0.3 J contact on the knee joint) and per os administration of diclofenac sodium (8 mg/kg) and glucosamine hydrochloride (50 mg/kg) in the different sequences at intervals of 15 minutes. Microscopic examination of articular cartilage and biochemical blood serum analyses (glycoproteins, sialic acid, hondroyitynsulfat-, alkaline phosphatase) were carried out 28 days after the last injection of dexamethasone. Results: in all rats in the experimental groups structural irregularities in articular cartilage were insignificant compared with the control. In animals after application LILRinitially and subsequent pharmacotherapy, morphological indicatorsof chondrocytes biosynthetic activity were most pronounced, and levels of serum inflammatory markers and alkaline phosphatase activity -were significantly lower than in the control and other research groups. Conclusion: complex application of LILR with subsequent administration of diclofenac sodium glucosamine hydrochloride results in the most complete recovery of quantitative and structural-metabolic state of chondrocytes of articular cartilage and helps to normalize metabolic biochemical markers of cartilage and bone tissue.
Highlights
The effectiveness of conservative treatment in patients with knee joint arthritis can be enhanced through the use of the linked pharmacological and physical factors
The possibility of comprehensive use of low-intensity laser radiation (LILR) chondroprotectors and nonsteroidal anti-inflammatory drugs in patients with early knee joint arthritis- depending on the consistency of their application is not well studied
Knee joint arthritis stage I modeled by triple dexamethasone injections (7 mg / kg / m) performed once a week
Summary
Экспериментальные исследования проведены на 40 белых крысах (возраст 6 мес., масса тела 250–300 г) популяции экспериментально-биологической клиники ИППС им. проф. Использовали аппарат «Мустанг» с лазерной насадкой ЛО2: длина волны 0,89 мкм, импульсная мощность 7–8 Вт, частота 3000 Гц, длительность сеанса 3 мин 42 с, что соответствовало дозе 0,3 Дж, которую рекомендуют применять для пациентов с указанной патологией. В более ранних наших исследованиях установлено, что первые концентрации диклофенака натрия (после его введения животным per os) регистрировались в плазме крови крыс уже через 15 мин [6, 7]. Из эксперимента крыс выводили путем декапитации через 3 и 28 суток после воспроизведения модели гонартроза (последней инъекции дексаметазона). Ультраструктурную организацию суставного хряща исследовали с помощью трансмиссионного электронного микроскопа ЕМВ-100 БР после соответствующей обработки материала и изготовления ультратонких срезов [17]. С помощью биохимических методов в сыворотке крови крыс анализировали содержание маркеров воспаления — гликопротеинов Изменение показателей считали статистически значимым при р < 0,05 [23]
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