Abstract
Summary A simple phosphatase method, utilizing only one buffer substrate concentration and one precipitant concentration for all dairy products, is presented in detail. In this test the dairy product is incubated in a highly concentrated Na 2 CO 3 -NaHCO 3 buffer substrate at pH 9.5 to 9.7 and followed by precipitation with a trichloroacetic-HC1 solution. The filtrate then is made alkaline with Na 2 CO 3 and color development proceeds using BQC. Overnight incubation is standard for this test but both a short (1-hr.) and a rapid (10-min.) variation are available whenever circumstances dictate and are practically interchangeable as regards technique. This universal dairy products phosphatase test is highly sensitive and accurate but many other advantages are indicated by its simplicity of operation, low cost, ability to produce accurate results on samples of unknown age or origin and relatively low control values. Results in this study point to the importance of using overnight incubation for greatest sensitivity and accuracy on all phosphatase tests utilizing BQC as a color developer.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
