Abstract
Aim:The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) used as a surgical adjunct to maintain the inflammatory process and to potentiate tissue healing, make the subject of recent research in regenerative medicine. This study was designed to evaluate the healing activity of PRP by its topical application on the skin experimentally injured in a sheep model.Materials and Methods:The study was conducted on 9 adult and clinically healthy males sheep. PRP was obtained by a protocol of double centrifugation of whole blood from each animal. After sterile skin preparation, full-thickness excisional wounds (20 mm x 20 mm) were created on the back of each animal. The animals were randomly divided into three equal groups of three sheep for each. In Group I, the wounds were treated with PRP, in Group II; wounds were treated with Asiaticoside; in Group III, wounds were treated with saline solution. The different treatments were administered topically every 3 days. Morphometric measurements of the contraction surface of the wounds and histopathological biopsies were carried out at the 3rd, 7th, 14th, 21st, and 28th days of healing.Results:The results of the morphometric data obtained revealed that it was significant differences recorded at the 7th and 14th day of healing in favor for animals of Group I. Semi-quantitative histopathological evaluation showed that PRP reduces inflammation during 3 first days post-surgical and promotes epithelialization in 3 weeks of healing.Conclusion:We concluded that topical administration of PRP obtained by double centrifugation protocol could potentially improve the skin healing process in sheep.
Highlights
The development of bioactive surgical adjuvant capable of maintaining the inflammatory process and potentiating healing is one of the great challenges of regenerative medicine
In Group I, the wounds were treated with platelet-rich plasma (PRP), in Group II; wounds were treated with Asiaticoside; in Group in Groups (II) and (III), wounds were treated with saline solution
We concluded that topical administration of PRP obtained by double centrifugation protocol could potentially improve the skin healing process in sheep
Summary
The development of bioactive surgical adjuvant capable of maintaining the inflammatory process and potentiating healing is one of the great challenges of regenerative medicine. Several studies have been conducted but widely controversial, the main divergence of which is the absence of a consensus that. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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