The Effectiveness of Plantaricin IIA-1A5 Powder Application to Extend the Storage of Fresh Cow’s Milk

Abstract

Abstract Bacteriocins are peptides that have antimicrobial activity that are non-toxic, non-allergenic, heat-resistant and have broad bactericidal activity. Bacteriocin that has been declared safe by the FDA (Food and Drug Administration) and has been widely used in the food industry is nisin, which is mainly used in fruit products, dairy and meat processing industries. Bacteriocin from L. plantarum IIA-1A5 called plantarcin IIA-1A5, using whey growth media enriched with skim milk, has effectiveness as an antimicrobial against pathogenic bacteria E. coli and S. aureus. In this study, the application of plantaricin IIA-1A5 was conducted for fresh cow’s milk products from dairy cows on smallholder farms. The purpose of this study was to evaluate the effectiveness of the application of plantarcin IIA-1A5 powder using a certain dose to extend the storage period of fresh cow’s milk. Plantaricin IIA-1A5 powder was prepared with M1 concentration level of 5.58 g mL-1 and M2 concentration 11.17 g mL-1, then stored at frozen temperature. Nisin as a positive control was prepared at 11.17 g mL-1. Fresh milk that has been given treatment is then stored at room temperature for up to 6 hours and observed every 2 hours, namely at 0, 2, 4, and 6 hours of room temperature storage. The result showed that physicochemical quality of fresh milk did not change during treatments. Plantarisin IIA-1A5 was able to suppress the growth of total microbes at room temperature for two hours. The dose of plantaricin IIA-1A5 is effective for use in smallholder dairy farms or using non-cooled dairy machines at concentration of 11.17 g mL-1.