Abstract
Abstract. Soils are increasingly threatened by spillage of petroleum products such as petrol, diesel fuel, gasoline at oil refineries, underground storage tanks and pump stations pipelines. This study aims to investigate the effectiveness of Pseudomonas putida as oil-biodegradable agent in soil contaminated with diesel (D100). The effectiveness on bioremediation have been conducted by examined several physico-chemical tests on diesel-contaminated soil before and after seeding with P. putida. The spillage stimulation of D100 was conducted at laboratory scale for 24 days of incubation time. The results show that the bioremediation treatment able to remove up to 82%, 55%, 48%, and 34% of nitrogen, total organic carbon, phosphate, sulfate and, respectively. The pH of soil sample was changed from pH 7.8 (Day 0) to 6.78 (Day 24) after the treatment. Meanwhile, the moisture content in the sample has increased from 39% (Day 0) to 59% (Day 24). All of these results show the good indication of quality improvement of polluted soil after treated with P. putida. It is apparent from the acquired results that the application of P. putida is suitable as effective microorganism and potentially exploits as useful diesel-soil biodegradable agent in polluted soil.
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