Abstract
Periodontitis is a biofilm-induced chronic inflammatory. The current therapy of periodontitis is scaling root planning and curettage, and followed by administration of antibiotic such as combination of Amoxicillin and Clavulanic acid. Hematopoietic Stem Cell has ability to differentiate into blood cells which is capable of homing and regenerating itself. Research Purpose: The aim of this study was to prove antibiotic and Hematopoietic Stem Cell administration can reduce TNF α expression. Method: This research was divided into four different groups, P1 as negative control group, P2 as positive control group which inoculated with P.gingivalis 109 for three weeks as chronic periodontitis rat model and USP were administered, P3 as chronic periodontitis rat model received Hematopoietic Stem Cell injection into the tail vein of rat, P4 as chronic periodontitis rat models were given Amoxicillin and Clavulanic acid 250 mg/kg BW orally then followed by Hematopoietic Stem Cell 106 injection into the tail vein of rat. After two weeks rat were sacrified and immunohistochemically analysed for expression of TNF α. The data were analysed by using Non-Parametric Test. Result: TNF α expression of negative control group was different as compared to positive control group and treatment group which given Hematopoietic Stem Cell injection, but there is no difference between negative control group with treatment group which given Amoxicillin and Clavulanic acid 250 mg/kg BW orally and followed by Hematopoietic Stem Cell injection. Clonclusion: The administration of Amoxicillin and Clavulanic acid 250 mg/kg BW and Hematopoietic Stem Cell can reduce TNF α expression on periodontitis rat model.Keyword: Hematopoetic Stem Cell, TNF α, Chronic Periodontitis, Antibiotic
Highlights
This research was divided into four different groups, P1 as negative control group, P2 as positive control group which inoculated with P. gingivalis 109 for three weeks as chronic periodontitis rat model and NaCl (USP) were administered, P3 as chronic periodontitis rat model received Hematopoietic Stem Cell injection into the tail vein of rat, P4 as chronic periodontitis rat models were given Amoxicillin and Clavulanic acid 250 mg/kg BW orally followed by Hematopoietic Stem Cell 106 injection into the tail vein of rat
Positive control group(P2) which was innoculated by P. gingivalis 109 showed firm tumor necrosis factor (TNF) α expression with chocolate coloured cementocyte as compared to P3 group, chronic periodontitis rat model received Hematopoietic Stem Cell 106 injection
TNF α expression of P4 group as chronic periodontitis rat models were given Amoxicillin and Clavulanic acid 250 mg/kg BW orally followed by Hematopoietic Stem Cell 106 injection into the tail vein of rat were negatively expressed
Summary
Research Purpose: The aim of this study was to prove antibiotic and Hematopoietic Stem Cell administration can reduce TNF α expression. Clonclusion: The administration of Amoxicillin and Clavulanic acid 250 mg/kg BW and Hematopoietic Stem Cell can reduce TNF α expression on periodontitis rat model. Recruited neutrophils to the gingival crevice fail to control a dysbiotic microbiota, which can invade the connective tissue and interact with additional immune cell types, such as macrophages (Mw), dendritic cells (DCs), and gd T cells; a subset of innate-like lymphocytes These cells produce proinflammatory mediators [such as the bone-resorptive cytokines tumor necrosis factor (TNF), interleukin (IL)-1b, and IL-17] and regulate the development of T helper (Th) cell types, which contribute to and exacerbate the inflammatory response (George, 2014)
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