THE EFFECTIVENESS OF AMNIOTIC MEMBRANE DEPENDING ON THE CAUSE OF CORNEAL DAMAGE IN DOGS

Abstract

The results of studies on clinical cases of keratitis in dogs of different etiology, using measuring instruments and visual methods, after transplantation, amniotic membrane, which is the inner of the three amniotic membranes, transparent, avascular, developing from the fetal ectoderm and consisting of epithelial layer, located on the main membrane and connective tissue stroma. These results make it possible to analyze and further study the effect of allogeneic mesenchymal stem cells in amniotic membrane transplantation in various defects of the eye in general, as well as corneas with ulcers of various etiologies . Eye injuries and chemical burns are ophthalmic emergencies that require immediate diagnosis and treatment, as they can lead to deterioration and loss of vision. Clinical manifestations of such burns are caused by exacerbation of the innate immune response due to infiltration of inflammatory cells and activation of stromal fibroblasts. New treatments are emerging that address restorative mechanisms that improve the surface of the eye after injury; for example, stem cell transplantation (SC) has been successfully reported for this purpose . ( Stem Cells Translational Medicine 2018;7:906–917 ). The main task of the study is to prove the effectiveness of the amniotic membrane to indicate: activation of proliferation, stopping tissue apoptosis, reducing the activity of the inflammatory process, accelerating epithelialization and normalization of the corneal stroma, which leads to normal restoration of transparency without vascularization and turbidity. Also factors due to the effect of specific placental properties that regulate metabolism in tissues and embryonic leaves of the fetus, which have the ability to regenerate tissues and to activate their own stem cells, regardless of the cause of damage. Key words : clinical cases of keratoconjunctivitis, dogs of different breeds, mesenchymal stem cells (MSCs), amniotic membrane transplantation (TAM), corneal condition