Abstract
In recent decades it is found that carotenoids have anticarcinogenic, cardioprotective, anti-atherogenic properties, and immunomodulating, radioprotective and anti-toxic effects. Discovered effect of carotenoids and lycopene, in particular, can be used to develop recommendations for prevention and treatment of those who are subjected to emergency situations of radiation and biological nature. This paper deals with spectrophotometery method for determining relative content of carotenoids in tomatoes of different ripeness levels. It is demonstrated that a wide complex absorption band within the wavelength ranging from 3500 to 6500 Å is superposition of absorption bands for specific carotenoid pigments, which are a part of in vivo tomato pulp samples. According to additivity law of absorbency, the area under obtained curves represents value directly proportional to the thickness of layer under investigation and total concentration of carotenoids that allows to identify relative content of carotenoids in examined samples. It was concluded that developed method of samples preparing for measurements and the spectrophotometery method itself for determining the relative content of carotenoids in research tomatoes, as well as waste raw materials and oil cake, has shown a sufficient reliability, high speed and economic viability.
Highlights
Carotenoids, or carotenoid pigments, form one of the most numerous and very common groups of natural pigments that cause the color of many plants and animals
In the human body carotenoids play an important role as initial products from which the vitamins of group A are formed
Carotenoids are used in medicine as physiologically active compounds, a source of vitamins, etc
Summary
Carotenoids, or carotenoid pigments, form one of the most numerous and very common groups of natural pigments that cause the color of many plants and animals. By their chemical nature, they rate as tetraterpenes class. It is known about 600 carotenoids, among which lycopene is especially noteworthy, which gives the red color to fruits of tomato, and it is commonly known that the color of the fruit is an important indicator of their quality and biological value. The discovered effect of carotenoids and, in particular, lycopene can be used to develop recommendations for the prevention and treatment of those who are subjected to the emergency situations of radiation and biological nature
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