The Effect Recearch of Laser Radiation with
 Wavelenght 1270 nm on the Specific Binding of IgG to S. Aureus Antigens and on the Ability of Staphylococcal Enterotoxin A to Induce a Specific Immune Response in Mice

Abstract

Relevance: Recently, laser radiation in the near infrared region of the spectrum has been increasingly used in medicine, coinciding with the absorption bands of endogenous molecular oxygen for the purpose of direct optical generation of its active form – singlet oxygen (light-oxygen effect). However, the mechanisms of its manifestation and interaction with biological objects have not been fully elucidated.
 Clarifying them is relevant and can serve as a basis for expanding clinical application.
 Purpose: To study the effect of laser radiation with l~1270 nm on the specific binding of IgG to antigens and the ability of staphylococcal enterotoxin to induce an immune response.
 The objectives of the work are to determine the exposure doses that can affect this process and to establish the very fact of this possibility.
 Materials and methods: Experimental laser device l~1270 nm (New Surgical Technologies, Moscow), staphylococcal antigen, staphylococcal enterotoxin A, blood serum of immunized mice.
 Results: Establishment of the fact of suppression of the antigen + antibody reaction in vitro, both during antigen irradiation and serum irradiation, as well as suppression of the ability of the irradiated enterotoxin to cause an immune response.
 Conclusion: Suppression of immune reactions under the influence of laser radiation with the listed substrates has been established, which may indirectly indicate the influence of singlet oxygen on the conformational structure of protein molecules. This fact is important for understanding one of the ways to implement the “light-oxygen effect” and can be used in the clinic.