The effect on NK cells immunity in regular whole blood and platelet donors

Abstract

Objective To analysis NK cell count and cytotoxic activity in regular whole blood and platelet donors and to evaluate their immunity. Methods The NK cell count was analyzed by flow cytometer in peripheral blood in three crowds, viz free regular whole blood, platelet, and the first time whole blood donors. The last crow was the control group. The NK cell cytotoxic activity was analyzed by mixed culture of effecter cell/ target cell. Results The NK cell numbers and its anti-ratio to target cells in free regular whole blood donors had no significant differences with the control group(P>0.05). The platelet donors' NK cell numbers was significantly increased compared to control group(P 3 months), and have enough time to recovery their NK cell numbers and cytotoxicity. In contrast, platelet donation interval is shorter than other groups, so they must increase their NK cell numbers to regulate the effect caused by low function. Key words: Regular donation; NK cell; Immune function; cytotoxicity; Flow Cytometer