Abstract
THE EFFECT OF ZINC-HYDROCHLORIC ACID HYDROLYSIS ON THE ESTROGENS IN HUMAN URINE
Highlights
ON THE ESTROGENS IN HUMAN URINE*Per cent of zinc dust and 15 volumes per cent of hydrochloric acid resulted in a marked increase of estrogen potency over that acquired by optimum conditions of simple acid hydrolysis; namely, 10 minutes boiling with 15 volumes per cent of hydrochloric acid
We present data on the effects of the hydrolytic processas derived by our procedure for the fractionation and photometric estimation of urinary estrogens [8] which show that zinc-hydrochloric acid hydrolysis of the estrogens in human urine (a) converts the major portion of estrone to estradiol, [5] yields estradiol titers which are higher than can be accounted for by mere conversion of estrone to estradiol, and (c) yields estriol titers
Each urine aliquot after hydrolysis was processedin identical fashion, so that any difference in estrogen titer must be attributed to the hydrolytic techniques under investigation
Summary
Per cent of zinc dust and 15 volumes per cent of hydrochloric acid resulted in a marked increase of estrogen potency over that acquired by optimum conditions of simple acid hydrolysis; namely, 10 minutes boiling with 15 volumes per cent of hydrochloric acid. They were unable to account for all of the increased estrogenic potency by conversion of estrone to a-estradiol and more complete hydrolysis and they postulated the occurrence in human urine of certain non-estrogenic compounds closely related to the estrogens (estrone, a-estradiol, and estriol), which were rendered estrogenic by hydrogenation [2].
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