Abstract
The effects of wounding on riboflavin status of the rat were investigated. Male Sprague Dawley rats were intramuscularly injected in the hind limb with lambda-carrageenan. Four groups were studied: ad libitum control (C); wounded (W); pair-fed to wounded (P); and pair-fed to wounded supplemented to receive 60μg riboflavin daily (R). All groups were fed a 15% casein diet containing 2 μg riboflavin per g diet. Decreased food intake in the W and P groups was associated with weight loss, less positive nitrogen and riboflavin balance, and increased erythrocyte glutathione activity coefficients (ERG-AC). In spite of restricted food intake, R group values were closer to C group for riboflavin balance and ERG-AC, suggesting that these parameters are affected primarily by riboflavin intake. Nitrogen balance improved but did not reach control values. Liver riboflavin was significantly lower in the W group when compared to all other groups (p<0.05) indicating an effect of stress on liver riboflavin utilization or storage.
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