Abstract
Background: The aim of this study was to investigate the effect of whey protein supplementation on myofibrillar protein synthesis (myoPS) and muscle recovery over a 7-d period of intensified resistance training (RT). Methods: In a double-blind randomised parallel group design, 16 resistance-trained men aged 18 to 35 years completed a 7-d RT protocol, consisting of three lower-body RT sessions on non-consecutive days. Participants consumed a controlled diet (146 kJ·kg−1·d−1, 1.7 g·kg−1·d−1 protein) with either a whey protein supplement or an isonitrogenous control (0.33 g·kg−1·d−1 protein). To measure myoPS, 400 ml of deuterium oxide (D2O) (70 atom %) was ingested the day prior to starting the study and m. vastus lateralis biopsies were taken before and after RT-intervention. Myofibrillar fractional synthetic rate (myoFSR) was calculated via deuterium labelling of myofibrillar-bound alanine, measured by gas chromatography-pyrolysis-isotope ratio mass spectrometry (GC-Pyr-IRMS). Muscle recovery parameters (i.e., countermovement jump height, isometric-squat force, muscle soreness and serum creatine kinase) were assessed daily. Results: MyoFSR PRE was 1.6 (0.2) %∙d−1 (mean (SD)). Whey protein supplementation had no effect on myoFSR (p = 0.771) or any recovery parameter (p = 0.390–0.989). Conclusions: Over an intense 7-d RT protocol, 0.33 g·kg−1·d−1 of supplemental whey protein does not enhance day-to-day measures of myoPS or postexercise recovery in resistance-trained men.
Highlights
Over recent decades, the role that nutrition and/or exercise has on muscle protein synthesis (MPS) has been guided by the use of isotopically labelled amino acid tracers in acute lab-based studies.Nutrients 2020, 12, 845; doi:10.3390/nu12030845 www.mdpi.com/journal/nutrientsit is known that a single bout of resistance-exercise training (RT) stimulates a postexercise increase in mixed-muscle and myofibrillar protein synthesis [1,2,3]
When high-quality dietary protein (i.e., rapidly digested and absorbed protein, rich in essential amino acids (EAA)) is ingested, in temporal proximity to resistance training (RT), a further synergistic increase in myofibrillar protein synthesis (myoPS) occurs, which can be sustained for 4 to 5 h postingestion [4,5,6,7]. This acute increase in myoPS is thought to facilitate muscle reconditioning and net protein accretion, which is important for individuals who engage in prolonged periods of intense RT
There were no differences between groups for: age, stature, body mass, lean body mass (LBM), LBM index (LBMI), body fat %, back-squat 1RM or RT-experience (p > 0.276)
Summary
The role that nutrition and/or exercise has on muscle protein synthesis (MPS) has been guided by the use of isotopically labelled amino acid tracers in acute lab-based studies.Nutrients 2020, 12, 845; doi:10.3390/nu12030845 www.mdpi.com/journal/nutrientsit is known that a single bout of resistance-exercise training (RT) stimulates a postexercise increase in mixed-muscle and myofibrillar protein synthesis (myoPS) [1,2,3]. When high-quality dietary protein (i.e., rapidly digested and absorbed protein, rich in essential amino acids (EAA)) is ingested, in temporal proximity to RT, a further synergistic increase in myoPS occurs, which can be sustained for 4 to 5 h postingestion [4,5,6,7] Following exercise, this acute increase in myoPS is thought to facilitate muscle reconditioning (i.e., repair and remodeling) and net protein accretion, which is important for individuals who engage in prolonged periods of intense RT. The aim of this study was to investigate the effect of whey protein supplementation on myofibrillar protein synthesis (myoPS) and muscle recovery over a 7-d period of intensified resistance training (RT).
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