The Effect of Vitamin E Administration in Topical Olive Oil and Metamizole Injection on Plasminogen Activator Inhibitor-1 (PAI-1) Peritoneal Fluid and Degree of Adhesion

Abstract

Background and Purpose: Post-laparotomy adhesion is a problem for surgeons as well as patients. The purpose of this study was to establish a decrease in PAI-1 levels of peritoneal fluid, the degree of adhesion macroscopic and microscopically in male wistar rats subjected to ileum abrasion, between the combination of administration of vitamin E in topical olive oil and injection of Metamizole, single or unstained. Method: Laboratory experimental research. Using 24 male wistar rats which were subjected to ileum abrasion Re-laparotomy was performed 14 days later to see the PAI-1 level of the peritoneal fluid, the degree of adhesion macroscopic and the degree of adhesion microscopically. Dataanalysis by ANOVA, Kruskal Wallis, and Spearman test. Result: There was a significant difference in peritoneal fluid PAI-1 levels in the P0-P3 group (p=0.036), but there was no significant difference in peritoneal fluid PAI-1 levels between P0-P1 (p=0.761) and P0- P2 (p= 0.113). There was a significant difference in the P0-P3 group (p=0.005), but there was no significant difference between P0-P2 (p=0.403) and P0-P1 (p=0.187). Correlation test showed a significant correlation between PAI-1 levels of peritoneal fluid and the degree of macroscopic intraperitoneal adhesion with a large correlation of r = 0.750 (macroscopic) and macroscopic intraperitoneal adhesion with a large correlation of r = 0.729 (microscopic).Conclusion: Combination of vitamin E in topical olive oil with metamizole decrease in PAI-1 levels in peritoneal fluid, macroscopic and microscopic degree of adhesion.