The effect of vincristine on morpho-functional state of neurons of cerebrospinal sensitive ganglions in experiment

Natalia Luchkiv

doi:10.15587/2519-8025.2017.93979

Abstract

Vincristine is a preparation of vegetable generation, gotten from the vegetable rose myrtle (Vinca rosea L.), belongs to the group of chemotherapeutic preparations with expressed neurotoxicity. The active use of vincristine in the treatment of Malignant tumors, especially in child age, conditions the study of influence of this preparation on peripheral nervous system. In this connection we studied morpho-functional state of neurons and gliocytes of rat cerebrospinal ganglion under effect of vincristine in experiment.Aim of the work – to study the morpho-functional state of neurons and gliocytes of sensitive cerebrospinal rat ganglion under effect of vincristine in experiment.Task of research – to reveal the disorder of structural components of sensitive cerebrospinal ganglion, conditioned by vincristine use.Methods of research. The complex of light optical, morphometric and electromicroscopic methods of research was used to reveal the effect of vincristine use on structural components of sensitive cerebrospinal ganglion. The experimental study was carried out on 31 rats of both sexes (16 – experimental and 15 control ones) with mass 200,0–220, 0 g. The animals were kept in vivarium with free access to the food and water according to bioethics requirements as to the treatment of experimental animals, testified by correspondent act of bioethical expert opinion.Results of research. The features of vincristine use in oncology for treating patients with Malignant tumors of different organs and systems of organism and also the side effects of this preparation were considered. On the base of gotten electromicroscopic data we established the vincristine effect on the nervous structures of sensitive cerebrospinal ganglion in pathogenesis of vincristine-induced peripheral neuropathy. Thus, during the experiment there was observed the expressed sensitivity of neurons of sensitive cerebrospinal ganglions to the toxic effect of preparation. The big neurons suffer most, the small ones – least.Conclusions. It was established, that vincristine effect is manifested by the disturbance of neurotubes and neurofilaments in esodic neurons with lesion of protein-synthesizing organobodies

Highlights

Vincristine belongs to the group of preparation of vegetable generation, gotten from the vegetable rose myrtle (Vinca rosea L.)
Materials and methods The complex of light optical, morphometric and electromicroscopic methods of research was used to reveal the effect of vincristine use on structural components of sensitive cerebrospinal ganglion
On the base of aforesaid we come to the conclusion that the effect of vincristine is manifested by disorder of neurotubes and neurofilaments in esodic neurons with lesion of protein-synthesizing organobodies

Summary

Introduction

Vincristine belongs to the group of preparation of vegetable generation, gotten from the vegetable rose myrtle (Vinca rosea L.) This preparation has cytostatic activity and causes the reverse mitotic block, binding preparation with cytoplasmatic precursors of mitotic spindle [1]. The active use of this preparation in treatment of malignant tumors, especially in child age, conditions the topicality of studying vincristine influence on peripheral nervous system. In this connection, we studied the effect of this preparation of morpho-functional state of neurons and gliocytes of cerebrospinal ganglion of rat for the first time

Methods

Results

Conclusion