The Effect of Various Training on the Expression of the 5’amp-Activated Protein Kinase Α2 and Glucose Transporter - 4 in Type-2 Diabetes Mellitus Rat

Abstract

BACKGROUND: Exercise is the main pillar in Type 2 Diabetes Mellitus (T2DM) management. The mechanism of glucose uptake mediated by exercise is different from insulin, and this mechanism is not disturbed in T2DM. One of the mechanisms is through the activation of 5’AMP-activated protein kinase (AMPK). AMPK also regulates the glucose transporter 4 (GLUT4) expression. Effect various types of exercise to AMPK α2 and GLUT-4 of the skeletal muscle still limited. AIM: This study aims to determine the effect of various physical training on the expression of Ampk α2 and Glut 4 in skeletal muscle of T2DM rats. METHODS: This study used stored skeletal muscles of 25 T2DM Wistar rats. Previously, the rats were divided into groups of K1 (control, not given exercise), K2 (moderate continuous training), K3 (severe continuous training), K4 (slow interval training), and K5 (fast interval training). Running on a treadmill frequency 3 times a week for 8 weeks. The relative expression of Ampk α2 and Glut 4 were assessed using Real Time-PCR and were compared among the groups using the Livak formula. RESULTS: Moderate intensity continuous training increased Ampk α2 and Glut 4 expression by 1.45 and 2.39 times, respectively. Severe intensity continuous training increased the expression of Ampk α2 and Glut 4 by 1.55 and 2.56 times, respectively. Slow interval training increased the expression of Ampk α2 and Glut 4 by 4.41 and 3.76 times, respectively. The expression of Ampk α2 and Glut4 in fast interval training was 4.56 and 4.79 times more than control. CONCLUSION: Continuous and interval training increase Ampk α2 and Glut 4 expression. The fast interval training showed the highest expression of Ampk α2 and Glut 4.