Abstract
At room temperature and pH 8.5, the effects of concentrated solutions of urea, nicotinamide, guanidine, sodium benzoate, and sodium salicylate upon the haemoglobins of adult and cord blood have been followed spectrophotometrically. In all cases the reaction curves were similar to those of alkali denaturation, but differed in the rates of reaction and failure to show any marked difference in rate between adult and cord blood. In no instance was the rate for cord blood greater than the corresponding rate for adult blood. 'The velocity constants for these experimental conditions have been calculated.Except in the case of the nicotinamide reaction, absorption curves of the reaction products before and after reduction with sodium dithionite showed differences from the corresponding curves of alkali denaturation at pH 11.9. It is suggested this may indicate varying degrees of denaturation.
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