The Effect of Ultraviolet Light on Microbial Inactivation and Quality Attributes of Apple Juice

Abstract

Non-thermal technologies such as UV irradiation can offer advantages for minimal processing of transparent beverages. In this study, reconstituted apple juice was exposed to UV light in a continuous laboratory scale system at energy dosages ranging from 2.66 to 53.10 J/cm2 by changing the exposure time. Treated juices were then evaluated for microbial inactivation and selected physical and chemical attributes. Product quality was further assessed by sensory evaluation using a 30-member consumer panel. Microbiological analysis was performed by inoculating apple juice with Escherichia coli K12 and Listeria innocua and microbial numbers were counted pre- and post-processing. UV energy levels did not affect pH, °Brix, or total phenols content, but decreased non-enzymatic browning (p < 0.01) and antioxidant capacity (p < 0.05) compared to unprocessed juice. A colour-lightening effect was noted with increasing energy dose. All UV treatments applied (2.66 J/cm2 and above) resulted in a reduction below the detection level (<1 log cfu/ml) for both E. coli and L. innocua in apple juice. Sensory evaluation showed that samples treated with energy dosages up to 10.62 J/cm2 were comparable to the control in terms of acceptability, though higher dosages produced adverse effects in terms of flavour and colour. Based on these results, UV treatment with low energy dosages could represent a valid alternative to thermal processing to eliminate pathogenic microorganisms while maintaining quality in reconstituted apple juice.