The Effect of Tuna Extract on Periarticular Tissue Catalase Activity and Malondialdehyde Levels in Animal Models of Adjuvant Arthritis

Abstract

Tuna contains vitamins, minerals, amino acids, and fatty acids, which play a role in preventing oxidative stress. The pathophysiology of rheumatoid arthritis is often associated with oxidative stress due to increased ROS production. Adjuvant arthritis experimental animals are widely used for research on rheumatoid arthritis. This study aims to determine the effect of tuna fish extract on plasma malondialdehyde (MDA) levels and periarticular tissue catalase in adjuvant arthritis (AA) experimental animals with AA. The 24 experimental animals were divided into 3 groups, group 1, eight experimental animals without treatment; group 2, eight experimental animals AA-induced complete freund adjuvant (CFA); group 3, eight experimental animals AA treated with Tuna fish extract therapy, and at the end of the study the animals were sacrificed and examined for plasma MDA and periarticular tissue catalase levels. The results of the ANOVA statistical test showed a significant increase in periarticular tissue catalase activity (p=0.001) between the AA group and the treatment group. There was a considerable decrease in periarticular tissue MDA levels (p=0.001) between the adjuvant arthritis group and the treatment group with p value = 0.001. The Spearman correlation test between MDA levels and catalase activity shows -0.335, indicating an adequate and inverse correlation. The administration of tuna extract can prevent oxidative stress. It can be shown that increasing the antioxidant activity of catalase and reducing lipid peroxidation is measured by examining MDA levels.