Abstract
The incorporation of [ 14C]leucine, in vivo, into proteins of maternal liver and placenta of mice 15 days pregnant, is inhibited by trypan blue. The effect on placental protein synthesis is noticeable earlier and persists longer in comparison with that on hepatic protein synthesis. On the other hand, hepatic protein synthesis is affected by lower doses than placental protein synthesis. All the subcellular fractions of maternal liver show reduction in protein synthesis whereas only the mitochondrial fraction of placenta shows inhibition. Amino acid incorporation into proteins of both liver and placenta of mice starved for 24 h is considerably reduced by trypan blue. Polysome disaggregation due to trypan blue is greater in liver than in placenta. Labelled leucine incorporation into free and membrane-bound ribosomal proteins is inhibited in both maternal liver and placenta.
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