The Effect of Triton WR‐1339 on Lipid Metabolism in Mice

Abstract

AbstractThe hyperlipemia and hypercholesterolemia were induced in mice by injection (i.p.) of a single dose of Triton WR‐1339 at a dosage of 900 mg/kg body weight. These animals were characterized by significant increases in the average levels of plasma total lipids, cholesterol, phospholipids and triglycerides. The highest rise of each fraction was after 24 hr. of triton injection. Plasma total lipids and cholesterol levels were increased to 10‐fold and phospholipids to 8‐fold while triglycerides reached to 14‐fold when compared to control animals. This hyperlipemic state was associated with the appearance of fatty nodules in liver tissue. These nodules were found to be lipoproteins which were collected in bags by the liver cells. The blood glucose level was slightly elevated after triton injection while liver and muscle glycogen contents were highly increased and the highest levels were recorded after32 hr. of triton injection. Triton also reduced the activity of liver lipogenesis dehydrogenases. Maximum reduction in 6‐phosphogluconic dehydrogenase (6‐PGD) and malate dehydrogenase was found after 8 hr. of triton injection but found after 16 hr. for glucose‐6‐phosphate dehydrogenase (G‐6‐PD). After 24 and 32 hr. the enzyme activities were elevated but still below the normal levels. The mechanism of triton injection on lipid metabolism and turnover in mice was also discussed.