The effect of triptolide and triptolide loaded liposomes on proliferation and apoptosis to TFK-1 cells

Abstract

Objective To research the effect of triptolide (TP) and triptolide loaded liposome (TP-LP) on proliferation and apoptosis to human cholangiocarcinoma cell line TFK-1,and the inhibition of tumor growth so that we can evaluate the feasibility of liposome as TP nano-carrier.Methods Detected the effect of TP and TP-LP at different concentration level on proliferation,cell cycle and apoptosis to TFK-1 by methyl thiazolyl tetrazolium (MTT),clone formation assay and flow cytometry.In animal study,we constructed TFK-1 node mice xenograft mode to compare the inhibiting effect to mice xenograft of TP and TP-LP.Results TP and TP-LP inhibited proliferation of TFK-1 both time and dose dependent,and TP-LP was superior to TP (P ＜ 0.05).Both TP and TP-LP could block TFK-1 cells in G0/G1 phase and promote them to apoptosis.After treated by TP or TP-LP at 100nmol/L after 48 h,the percentage of G0/G1 phase were (73.26 ± 2.56) ％ and (84.35 ± 3.85) ％ seperately,and the percentage of apoptosis were (22.71 ± 3.66)％ and (35.23 ± 4.02) ％ separately,both of which had significant difference (P ＜ 0.05).The volume of xenograft in control,TP and TP-LP group were (1 073.33 ± 20.82),(306.67 ± 15.28) and (88.0 ±8.26) mm3 seperately,which had significant difference (P ＜0.05).While 21 days after drug-using,the weight of mice in three groups were (24.69 ± 1.53),(22.85 ± 1.99) and (22.80 ± 1.77) g separately,which had no significant difference (P ＞ 0.05).Conclusion Both of TP and TP-LP can inhibit proliferation of TFK-1 by blocking cell cycle and promoting apoptosis in vitro,and inhibit xenograft growth in vivo,while TP-LP is effective.So TP-LP is a potential new drug,which is more effective than TP. Key words: Triptolide ; Liposome ; Proliferation; Apoptosis ; Cholangiocarcinoma