Abstract
Hypoxic stress is a risk factor of ocular neovascularization. Hypoxia visualization may provide clues regarding the underlying cause of angiogenesis. Recently, we developed a hypoxia-specific probe, protein transduction domain-oxygen-dependent degradation domain-HaloTag-Rhodamine (POH-Rhodamine). In this study, we observed the localization of HIF-1α proteins by immunohistochemistry and the fluorescence of POH-Rhodamine on RPE-choroid flat mounts. Moreover, we compared the localization of POH-Rhodamine with pimonidazole which is a standard reagent for detecting hypoxia. Next, we investigated the effects of triamcinolone acetonide (TAAC) against visual function that was evaluated by recording electroretinogram (ERG) and choroidal neovascularization (CNV) development. Mice were given laser-induced CNV using a diode laser and treated with intravitreal injection of TAAC. Finally, we investigated POH-Rhodamine on CNV treated with TAAC. In this study, the fluorescence of POH-Rhodamine and HIF-1α were co-localized in laser-irradiated sites, and both the POH-Rhodamine and pimonidazole fluorescent areas were almost the same. Intravitreal injection of TAAC restored the reduced ERG b-wave but not the a-wave and decreased the mean CNV area. Furthermore, the area of the POH-Rhodamine-positive cells decreased. These findings indicate that POH-Rhodamine is useful for evaluating tissue hypoxia in a laser-induced CNV model, suggesting that TAAC suppressed CNV through tissue hypoxia improvement.
Highlights
The effect of triamcinolone acetonide on laser-induced choroidal neovascularization in mice using a hypoxia visualization bio-imaging probe
These findings indicate that PTD]-oxygen dependent degradation domain [ODD]-HaloTag (POH)-Rhodamine detected HIF-1a-positive cells in a laserinduced choroidal neovascularization (CNV) model
In the present study, we succeeded in imaging hypoxic conditions in the retinal pigment epithelium (RPE)-choroid complex using POH-Rhodamine in a laser-induced
Summary
The effect of triamcinolone acetonide on laser-induced choroidal neovascularization in mice using a hypoxia visualization bio-imaging probe. We investigated the effects of triamcinolone acetonide (TAAC) against visual function that was evaluated by recording electroretinogram (ERG) and choroidal neovascularization (CNV) development. The area of the POH-Rhodamine-positive cells decreased These findings indicate that POH-Rhodamine is useful for evaluating tissue hypoxia in a laser-induced CNV model, suggesting that TAAC suppressed CNV through tissue hypoxia improvement. To start early-stage treatment and prevent serious vision loss, it is necessary to detect hypoxic conditions in choroid and retinal pigment epithelium (RPE) of AMD patients. Glucocorticoids have anti-inflammatory effects and are widely used in clinical practice In ophthalmology, they are commonly used to treat of ocular pathologies associated with vascular leakage and ocular neovascularization[10,11]. Little has been reported on the effect of TAAC on HIF-1a expression in intraocular cells
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