The effect of triadimenol on the cytology and growth of sensitive and resistant strains of Ustilago avenue

Abstract

SUMMARYTwo triazole resistant mutants of Ustilago avenae and a wild type strain were treated with different concentrations of the fungicide triadimenol in liquid cultures. Growth rates were measured and the morphology of the sporidia examined by light and electron microscopy. After treatment with 1 – 10 μg ml‐1 for 4 days wild type sporidia increased in growth rate, changed from budding to filamentous growth and had considerably thicker cell walls. Their cytoplasm also showed strong degeneration and damage to the cell organelles. The resistant strains were not influenced under these conditions but under longer exposure (14 days) to higher fungicide concentrations (50 to 200 μg ml‐1) similar changes to growth rates, morphology and cell wall thickness also occurred. However, cell disruption only occurred at 200 to 250 μg triadimenol ml‐1 and there were differences in cell wall thickening. One resistant strain (r13) never showed thickening, while in the other (r8), the wall consisted of two distinct layers. The implications of these results are discussed in relation to possible resistance mechanisms.