The effect of transmembrane 7 superfamily member 4 gene on behavior of breast cancer cell

Abstract

Objective To investigate the effect of transmembrane 7 superfamily member 4 (TM7SF4) gene on behavior of breast cancer cell. Methods The TM7SF4 lentivirusis constructed and used to transfect MCF-7 human breast cancer cells, and then down-regulate TM7SF4 gene. Investigate the effect of TM7SF4 gene on the proliferation, apoptosis, cell cycle and cell proliferation of MCF-7 cells by Cellomics, flow cytometry, propidium iodide-fluorescence activated cell sorter (PI-FACS) and clone formation. Results (1) Effect on proliferation of MCF-7 cells: the number of MCF-7 cellsof experimental group in 5 days cultured were: (239.1±37.7), (508.4±52.8), (282.2±84.0), (308.0±167.1), (131.6±82.6) cell/well; the number of MCF-7 cells of no-treatment control group in 5 days cultured were: (382.8±38.0), (1 115.0±155.9), (1 426.2±192.4), (2 468.4±232.6), (2 903.0±165.7) cell/well; the number of MCF-7 cells of plasmid-treatment group in 5 days cultured were: (380.8±39.0), (1 090.0±145.9), (1 426.0±180.3), (2 379.0±228.6), (2 855.0±156.6) cell/well. results showed that the number of cells and rate of cells growth of experimental group were significantly inhibited (P<0.05); (2) Effect on cell cycle of MCF-7 cells: the number of MCF-7 cells of experimental group at G0/G1 stage, S stage, G2/M stage were (61.87±0.27), (20.31±0.11), (17.83±0.35) cell/well, respectively; and that of no-treatment control group were (57.46±1.40), (30.42±0.36), (12.83±0.55) cell/well, respectively, and that of plasmid-treatment group were (57.78±1.23), (30.11±0.47), (13.30±0.79) cell/well, respectively. Statistical differences were found between experimental group and no-treatment control group, between experimental group and plasmid-treatment group (P<0.05); (3) Effect on apoptosis of MCF-7 cells: MCF-7 cell apoptosis rate of experimental group, no-treatment control group and plasmid-treatment group was: (9.94±0.57)%, (5.88±0.23)%, (5.80±0.24)% in scatter diagram and (27.37±1.11)%, (23.34±0.50)%, (23.37±0.46)% in peak valuesdiagram, respectively; There were statistical difference between experimental and no-treatment control group, between experimental group and plasmid-treatment group both in scatter diagram (P<0.05) and peak values diagram (P<0.05), respectively; (4) Effect on clone formation of MCF-7 cells: number of the colony MCF-7 cells in the experimental group, no-treatment control group and plasmid-treatment control was 11±3, 34±2, 29±1, respectively. There were statistical difference between experimental group and no-treatment control group (P<0.05), between experimental group and plasmid-treatment control group (P<0.05), respectively. Conclusion It was confirmed that the TM7SF4 gene had the ability to promote the proliferation and clone formation of MCF-7 breast cancer cells, and to inhibit the apoptosis and cell cycle progression. TM7SF4 gene played a role in promoting the development of breast cancer cells. A new theoretical basis and experimental data were presented for early diagnosis and targeted therapy of breast cancer. Key words: Breast cancer; Transmembrane 7 superfamily member 4; Apoptosis; Proliferation