The Effect of Topical Gel Secretome Hypoxic Mesenchymal Stem Cells (SH-MSCs) on IL-6 and TGF- β Gene Expression (Experimental Study in Male Wistar Alopecia-Like Rat Models Induced by Fluconazole)

Abstract

Alopecia induced by the accumulation of reactive oxygen species (ROS) stimulates changes in the immune and neuroendocrine systems. Hair follicles have dermal papillary cells (DPCs) that bind to corticotropin-releasing factor (CRF) receptors. Research shows that transforming growth factor (TGF-β) is one of the expressions that can be an indication of the influence of CRF. The effect of CRF itself is to cause hair loss. The purpose of this study was to determine the Secretome of Hypoxia Mesenchymal Stem Cells (SH-MSCs) against IL-6 expression and TGF-β expression. This study used experimentally in vivo with the Post Test Only Control Group Design method. The subjects of this study were male rats of Wistar strain which were divided into 4 treatments consisting of 1 healthy group, 1 negative control group, 1 treatment group with SH-MSCs dose 10% in a dose of 200 mg/rat/day gel, and 1 more treatment group with SH-MSCs dose 20% in a dose of 200 mg/rat/day. On the 21st day, IL-6 expression and TGF-β expression were examined. Data were analyzed using the One-Way ANOVA Test and the Kruskall-Wallis Test to determine the effect of each group. The One-Way ANOVA test showed the results of IL-6 expression (p = 0.000) showed a significant difference (p < 0.05) and the Kruskall-Wallis test of TGF-β expression (p = 0.000) with SH-MSCs dose 200 was a significant difference (p < 0.05). The administration of 10% and 20% doses of SH-MSCs in 200 mg gel may decrease IL-6 expression. Dosed SH-MSCs 20%increased TGF-β expression in alopecia-like rats. The development of the use of SH-MSCs is expected to be an alternative to alopecia therapy that is more beneficial and safe for the body.