The effect of time of feeding on plasma amino acids during exercise and recovery in horses.

Abstract

Feeding management in horses suggests feeding horses in advance of exercise, particularly the grain portion of the diet. Plasma amino acids (AA) peak at 3 to 6 h postfeeding depending on the AA. The timeframe between feeding and exercise may affect the availability of AA during and after exercise. The purpose of this study was to observe the differences in plasma AA in horses fed prior to exercise or after exercise. Eight light type horses were fed a diet with adequate protein and AA for horses in light to moderate exercise. After an adjustment period, horses completed a standardized exercise test (SET). Relative to the SET, horses were fed either 2 h prior (PRE horses) to the SET, 1 h after completing the SET (POST horses), or horses remained fasted throughout the sampling period (FASTED horses). Plasma was drawn prior to exercise, at the peak of exercise as well as at 1, 2, 4, and 7 h postexercise. Plasma was analyzed for AA, glucose, lactate, creatinine, creatine kinase, ammonia, urea-N, and 3-methylhistdine. After completion of the SET and sampling period, horses entered a 1-wk recovery period, which was followed by another SET. The protocol repeated until horses rotated through all feeding protocols in the study (three SETs). The majority of the plasma AA were elevated in PRE horses compared with POST horses prior to the SET until 2 h postexercise where POST horses’ plasma AA concentrations became elevated and remained elevated until the end of the sampling period. In that same time frame, plasma AA for the PRE group decreased out to the end of the sampling period. The elevation of plasma AA in POST horses would be expected as they were fed at 1 h postexercise, whereas PRE horses were reaching a 4 h postfeeding time frame at this point. This elevation was not observed for plasma concentrations of isoleucine, leucine, methionine, and histidine. Concentrations of these AA initially were greater for POST horses in the postexercise period; however, they declined more rapidly than the other AA. The rapid decrease of some of the plasma AA concentrations may suggest uptake by muscle for recovery. This in conjunction with a decrease in plasma creatine kinase concentrations for POST horses suggests that feeding postexercise may facilitate better muscle protein balance (synthesis vs. breakdown) in the recovery period following exercise.