The effect of the surface charge of hydrogel supports on thermophilic biohydrogen production

Abstract

The effect of the surface charge of different immobilizing hydrogels on biohydrogen production in batch cultures was investigated using a novel isolate associated to the genus Thermoanaerobacterium. Two crosslinked polysaccharide-based hydrogels and two acrylic hydrogels were tested as polymeric carriers for cell adsorption. Immobilization improved both substrate conversion and hydrogen cumulative production compared to the suspended culture, and a yield of 1.9mol H2/mol glucose was observed after 24h for alginate-supported cultures. Cationic carriers dramatically increased cell immobilization, leading to markedly faster kinetics of substrate degradation and hydrogen production in batch operation, with a peak of 3.6mol H2/mol glucose for the acrylic hydrogel HM92. Accumulation of gaseous and acidic metabolites inhibited further H2 production, shifting the carbon flow to reduced end-products and biomass synthesis. Preliminary tests showed that all the tested hydrogels had good durability and allowed hydrogen production on repeated batch runs.