Abstract
We have investigated the use of five different promoter-5′UTR leader combinations to develop a potato plant-based expression system for the production of VP60 protein from rabbit haemorrhagic disease virus (RHDV). The relative efficiency of CaMV 35S, modified CaMV 35S, sunflower polyubiquitin and patatin promoters, as well as the addition of the 5′ leader sequence from φ10 gene of T7 phage, on VP60 gene expression in leaf and tuber tissues of plants, cultivated both in vitro and as potted plants, was investigated. Our results indicated that the B33 promoter with the φ10 leader gave the highest level of expression in tubers. The rabbits immunised with tuber plant extracts containing VP60 elicited specific antibody responses and were protected against challenge with virulent RDHV.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
