The effect of the hydrophilic spacer length on the functionality of a mercury-supported tethered bilayer lipid membrane

Abstract

A biomimetic membrane consisting of a thiolipid monolayer tethered to a mercury electrode, with a dioleoylphosphatidylcholine (DOPC) monolayer on top of it, was fabricated. The thiolipid, referred to as DPOL, consisted of an octaethyleneoxy (OEO) chain terminated at one end with a lipoic acid residue and covalently linked at the other end to two phytanyl chains. The functionality of this biomimetic membrane, referred to as a tethered bilayer lipid membrane (tBLM), was tested by incorporating gramicidin and alamethicin and verifying their ion channel activity. Advantages and drawbacks with respect to a tBLM using a thiolipid, referred to as DPTL, with a tetraethyleneoxy (TEO) chain were examined by using electrochemical impedance spectroscopy, potential-step chronocoulometry and cyclic voltammetry. The maximum charge surface density of potassium ions stored in the OEO spacer amounts to 70μCcm−2, as compared to a charge surface density of 45μCcm−2 in the TEO spacer. The lipid bilayer moiety of the DPOL/DOPC tBLM is somewhat leakier than that of the DPTL/DOPC tBLM at potentials negative of about −0.65V vs. the saturated calomel electrode. The estimated value of the surface dipole potential of the OEO spacer amounts to −0.180V and is, therefore, smaller than that, −0.230V, of the TEO spacer.