Abstract
Combined effect of aluminum chloride and quercetin solutions on the enzymatic activity and contraction dynamics of muscle fiber bundles of the Rana temporaria m. tibialis anterior was investigated. It was shown that these complexes inhibit muscle contraction. Linear reduction of Ca2+, Mg(2+)-ATPase activity induced by all of the used concentrations of AlCl3 - quercetin was demonstrated. It was found that complex of quercetin with AlCl3 has a greater inhibitory effect on muscle contraction dynamic and causes greater reduction during all periods of stimulation in comparison to the separate effect of the investigated compounds. All the studied concentrations of AlCl3 and quercetin solutions (AlCl3: 10(-4)-10(-2) M; quercetin: 10(-6)-10(-5) M) caused concentration depended contraction strengths and lengths reduction. The decrease in strength and length of muscle contractions was of constant and mostly linear nature within observed timeframe as well as within each periods of contraction. The changes were least pronounced within pretetanic period, but were profound within terminal period of muscle activity. The changes in dynamic contraction properties and Ca2+, Mg(2+)-ATPase activity of sarcoplasmic reticulum under effect of the investigated compounds was minimal in the beginning of the muscle's response to stimulus, prior to muscle strength reaching stable contraction level.
Highlights
Flavonoids, biologically active substances of natural origin, are effective as modulators of muscle contraction-relaxation cycle [1]
We studied changes in dynamic parameters of contraction of electrically stimulated isolated frog muscle fibers under non-cholinergic effect of investigated compounds [9], which improves considerably d. m. nozdrenko, o. m. abramchuk, v. m. soroca, n. s. miroshnichenko upon understanding of toxic effects of quercetin-aluminum complexes on skeletal muscle, since disruption in muscle function under the effect of such compounds is generally viewed as resulting from acetylcholinesterase inactivation
Since quercetin is capable of producing complexes with metals, in particular with aluminum, the aim of the present work was to investigate the effect of aluminum chloride solutions with quercetin on Ca2+,Mg2+-ATPase activity of sarcoplasmic reticulum (SR) and dynamics of electrostimulated muscle contraction of isolated thin muscle fibers
Summary
Flavonoids, biologically active substances of natural origin, are effective as modulators of muscle contraction-relaxation cycle [1]. Flavonoids are known to affect contractile systems of various biological objects They have been demonstrated to regulate activity of all types of muscles, including smooth, skeletal, and cardiac muscle [2, 3]. Aluminum-flavonoid complexes may be produced during cooking of vegetable food in an aluminum vessel and consumed [3] It has been shown [6], that flavonoid interaction with metal ions, e.g. aluminum, may change the flavonoid’s properties and biological effects. S. miroshnichenko upon understanding of toxic effects of quercetin-aluminum complexes on skeletal muscle, since disruption in muscle function under the effect of such compounds is generally viewed as resulting from acetylcholinesterase inactivation. Ca2+,Mg2+ATPaseactivity decrease probably results from compromised structural integrity of membranes of SR and not from direct effect of flavonoids and their metallic complexes upon the enzyme [10]. We primarily determined the timeframe for setting in of equilibrium stable state of contraction under the effect of the investigated compounds
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