The effect of Temulawak extract gel on angiogenesis and re-epithelization rate post-tooth extraction of Wistar rats

Abstract

Introduction: Post-extraction wound healing involves angiogenesis process followed by re- epithelialization. Temulawak is an herbal plant containing active substances such as curcumin, tannins and flavonoids, which could stimulate the formation of growth factors and encourage fibroblast migration. Further, it would facilitate angiogenesis and increase the migration of epithelial cells to accelerate wound healing process. Objective: to assess the effect of 5% temulawak extract gel on angiogenesis and the re- epithelialization rate after tooth extraction in Wistar rats. Methods: this study used in vivo laboratory experimental methods on 30 Wistar rats, divided into control and treatment groups. Mandibular left incisor was extracted and the Wistar rats were euthanized on the 3rd, 5th, and 7th day. The mandibles of the rats were then decapitated and observed under a microscope with 400x magnification. Data analysis was performed with SPSS Software. Results: LSD post-hoc analysis showed a significant difference between control group day-7 and treatment group day-3, as well as treatment group day-7 (p<0,05). There is also a non- significant difference in epithelial gap between control group day-5 and treatment group day-3, as well as between control group day-7 and treatment group day-5 (p>0,05). Conclusion: 5% temulawak extract gel has a statistically significant effect on angiogenesis and the re-epithelialization rate after tooth extraction in Wistar rats.