Abstract

Citrobacter intermedius was grown batchwise in a mineral salts medium with glucose as a carbon and energy source under anaerobic conditions and at different temperatures. The effect of temperature on the following parameters was studied: maximum specific growth rate, μ max, maximum rate of hydrogen gas production and maximum rate of total gas (H2 + CO2) production. Arrhenius plots were used to determine the activation energies associated with μ max and maximum rates of hydrogen gas production.

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