The effect of temperature and pollution concentration on Cypermethrin removal in liquid media by using Thiobacillus sp. and Clostridium sp.

Abstract

Cypermethrin is known as synthetic pyrethroid (SP) which works to interfere the nervous system with a molecular formula of C9H11C13NO3. The use of pyrethroid pesticides which tends to be high and continuous without regard to the rules of pest control can have a negative impact, that is decreasing environmental quality and decreasing biodiversity. Pollution degradation in the soil due to pesticide residues can be done biologically by doing remediation technology. One of the microorganisms that can degrade insecticide residues in the soil is bacteria. Thiobacillus sp. and Clostridium sp. are insensitive to cypermethrin which has been prove by no inhibition zone formed on a media that contained cypermethrin. Therefor this research the aim is to determine the growth response of bacterial culture using Thiobacillus sp. and Clostridium sp. as a bioremediation to remove the insecticide cypermethrin in liquid media. Optimization of environment conditions use temperature with a variation (°C) of 25-40 and concentration of cypermethrin with a variation (ppm) of 100-250 cypermethrin analysis using the Gas-Chromatography Mass Spectrometer (GC-MS). This research proves that the bacterium Thiobacillus sp. and Clostridium sp. capable of removing cypermethrin in liquid media reaching 97% occurs at optimum conditions at 30°C and optimum concentration of 100 ppm.