Abstract
Temperature and availability nutrients played an important role on growth and lipid production of microalgae. In this study, we examined the effect of increasing suhu and excessed and depleted NO3 on growth rate, biomass and free fatty acid concentration in the Spirulina sp and Skeletonema sp. Two microalgae were culture on Conway and Milne media for 21 days using continuous culture technique. There were four temperature treatments, such as 28oC, 30oC, 32oC and 34oC and three nutrient treatments, which were control nutrient treatment, without NO3 and two times NO3 concentrations from control treatments with three replicates for each treatments. Results found that increasing temperature significantly affected on biomass and concentration free fatty acid, meanwhile nutrient treatments affected on growth rate, biomass and concentration of organic Carbon. In general, increasing temperature was more affected on Spirulina sp in terms of increasing growth rate, biomass and free fatty acid concentration. However, Skeletonema sp was more responsive to availability of NO3 in the media culture for increasing free fatty acid, and percentage of free fatty acid per dry weight.
Highlights
Many countries in the world are rapidly
The price of fuel oil is being used as fuel, petroleum is a source of electrical skyrocketing and the security conditions of oil- energy
The purpose of this research is to know the effect of temperature increase and excess and deficiency of NO3 in culture medium to growth rate, biomass and free fatty acid content from microalgae Spirulina sp and Skeletonema sp
Summary
Many countries in the world are rapidly. Currently oil reserves in Indonesia are only experiencing two major problems, namely the 4.9 billion barrels. Situation exacerbates the economic conditions the petroleum reserves used for the especially of the developing countries To overcome those issues, it has become an requires a large amount of fuel oil. Energy urgent need to find alternative energy sources to crises such as fuel oil (BBM) and electrical energy solve the problem of rising fuel prices in the are becoming hot issues in Indonesia. Biofuel consists of several types, those are: Bioethanol, Biodiesel and Biogas These alternative energy sources come from plants (biomass). Biofuel research derived from mainland crops has been widely practiced, but biofuel research sourced from algae/microalgae in Indonesia is rarely conducted and published whereas the potential of microalgae as an alternative energy source is enormous. Several studies on the potential of microalgae as biofuel have been widely practiced in developed countries. While the focus of research is on the content of organic C and fatty acids from the test microalgae
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