Abstract
The frequency of 6-thioguanine resistant (TG r) mutant T-lymphocytes arising in vivo in humans can be quantified with a cell cloning assay. However, the in vivo proliferation of T-lymphocytes that may include TG r mutant cells can distort the relationship between mutation events and the resulting frequency of mutant cells. The T-cell receptor (TCR) gene rearrangement pattern of T-cell colonies can be used as an independent measure of clonality. Analysis of T-cell ‘clonality’ in 413 wild type and 1736 TG r mutant isolates from 58 individuals shows that mutant clonality is a frequent occurrence ( 35 58 individuals = 60.3% ). However, a major effect on the mutant frequency corrected for clonality (the calculated ‘mutation frequency’) was found only in nine samples all of which had mutant frequencies greater than 40 × 10 −6.
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