The effect of synthesized cartilage tissue from human adipose-derived mesenchymal stem cells in orthopedic spine surgery in patients with osteoarthritis.

Tang Weijie,Guan Xinhua,Yin Xiling,Han Jingqi,Feng Zuoji

doi:10.14715/cmb/2021.67.3.19

Abstract

Osteoarthritis is a joint disease that causes degeneration of articular cartilage and involvement of subcutaneous bone and inflammation of surrounding tissues. It can affect any joints, but the most common joints are the joints of the hands, feet, knees, thighs, and spine. Osteoarthritis patients need surgery in acute cases. The use of methods that increase the efficiency of this surgery has always been considered by researchers and surgeons. For this purpose, in the current study, the effect of synthesized cartilage tissue from human adipose-derived mesenchymal stem cells was considered in orthopedic spine surgery in patients with osteoarthritis. Thirty patients over the age of 60 who had acute spinal osteoarthritis and required surgery were selected. The pellet culture system of human adipose-derive mesenchymal stem cells of each patient was used to construct cartilage tissue. For 15 of them, in addition to implants, cartilage grafts were transplanted during surgery. All patients were monitored by the Oswestry Disability Index questionnaire, for one year. In general, the results showed that over time, patients with transplanted cartilage tissue and implants were in a better condition than patients who underwent only implant surgery.

Highlights

Osteoarthritis is a joint disease that first affects the cartilage of the joint
We aimed to investigate the effect of synthesized cartilage transplantation, from human adipose-derived mesenchymal stem cells, in the treatment of patients with osteoarthritis of the spine
Cartilage tissue synthesis During cell isolation and proliferation, adipose-derived mesenchymal stem cells grew as fibroblast-like or spindle-shaped with distinct nuclei and they were observed by contrast phase microscopy (BHS, OLYMPUS, USA)

Summary

Introduction

Osteoarthritis is a joint disease that first affects the cartilage of the joint. Cartilage is a slippery tissue that covers the ends of bones in a joint [1]. Bones owe their easy movement to healthy cartilage [2]. Another function of cartilage is to absorb impulses during physical movement [3]. The cartilage becomes thinner and gradually disappears in some areas, causing the bones to rub against each other, and there will be pain, inflammation, and decreased movement in the joint [4]. Unlike other joint inflammations, such as rheumatoid arthritis, osteoarthritis affects only the joints and does not affect other organs [6]

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