The effect of Survivin antioligonucleotide on the apoptosis of Xuanwei lung adenocarcinoma cell

Abstract

The aim of the study was to investigate the effects of Survivin antioligonucleotide on the proliferation, apoptosis and cell cycle of Xuanwei lung adenocarcinoma cell XWLC-05. Specific targeting Survivin ASODN was composed firstly. XWLC-05 would be divided into 4 groups: Group Sham (blank), Group Lip (simple liposome), Group Lip-SODN (transfected sense oligonucleotide) and Group Lip-ASODN (transfected ASODN). All groups had been transfected under the same condition for 48 hours. Then West blotting was used to check the expression of Survivin in cells from different groups and flow cytometer was used to find out the apoptosis rate of cells in different groups. The expression of XWLC-05 Survivin in Group Lip-ASODN decreased obviously and apoptosis rate was significantly higher than that of other groups (P < 0. 05). XWLC-05 transfected by Survivin ASODN could down regulate the expression of Survivin and lower expression of Survivin might lead to the apoptosis of XWLC-05 and restrain the proliferation of cancer cells.