The effect of surfactan on formulation and stability of nanoemulsion using extract of Centella Asiatica and Zingiber Officinale

Abstract

In this study a crude extract of Centella asiatica and ginger extract as bioactive compound on formulated nanoemulsion using sonicator at frequency 20 kHz, 500 Watt with 7-18 kHz ultrasonic wave frequency with emulsification time 10-60 minutes. The formulated nanoemulsion used oil-in-water emulsion type (O/W) weight ratio of 20:80 (% b/w) and Hydrophilic-Lypophilic Balance (HLB) values of the O/W emulsion system in the range of 10.0-16.5 to obtain emulsions on a nanometer scale. Surfactants used in this study were tween 85 and triethanol amine. The results showed that the optimum frequency at 7 kHz with nanoemulsion particle size of 222.9 nm with emulsification time 30 minutes. The effect of surfactant tween 85 on nanoemulsion showed that the higher concentration of surfactant tween 85, the smaller size of the nanoemulsion particle was. The concentration of tween 85 surfactant in the amoun of 8.0% weight was the optimum concentration with 99.2 ± 45.2 nm nanoemulsion particle size on HLB 13.93. The concentration of surfactant tween 85 (8.5 wt%) with HLB 14.50, the nanoemulsion particle size rose again to 126.1 ± 4.5 nm, while the optimum concentration of surfactant triethanol amine at 1.2 wt%, with the particle size of nanoemulsion 82.6 ± 46.5 nm.