The Effect of Supplementation with Some Essential Oils on the Mobility and the Vitality of Human Sperm.

Modou M Mbaye,Boutaina Addoum,Papa D Mar,Noureddine Louanjli,Bouchra El Khalfi,Brahim Saadani,Abdelaziz Soukri

doi:10.1155/2019/4878912

Modou M Mbaye, Boutaina Addoum + Show 5 more

Open Access

https://doi.org/10.1155/2019/4878912

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Journal: TheScientificWorldJournal	Publication Date: May 14, 2019
Citations: 5	License type: CC BY 4.0

Affiliation: University of Hassan II Casablanca

Abstract

The objective of this work is to study the improvement effect of some essential oils of sage (Salvia officinalis), oregano (Origanum vulgare), and eucalyptus (eucalyptus globulus) on the physiological parameters characterizing the quality of human sperm (mobility and vitality). We find natural biomolecules to improve sperm quality to increase the chances of success of very low in vitro fertilization (IVF) that stagnate around 20%. Sperm samples were mixed with different concentrations of essential oils. The effect of these essential oils on the motility and vitality of spermatozoa has been analyzed. The mobility was determined using a Computer Assisted Sperm Analysis (CASA). In the other side, the evaluation of sperm vitality was performed by staining eosin 2% and the microscopic examination is carried out via optical microscope. A drop of sperm will be mixed with a drop of eosin solution 2%, spread between the slip and coverslip, then allowed to air dry, and examined under a microscope. A significant improvement in the mobility and vitality of human spermatozoa has been noted with oregano. Eucalyptus after 10 min of exposure also significantly improves the mobility and vitality of the spermatozoa. Sage does not improve mobility for these incubation times but significantly improves vitality.

Highlights

Infertility is one of the new plagues of the 21st century and more and more couples are affected each year
Because the importance of these two parameters on the chances of conceiving in vitro fertilization (IVF) or naturally is no longer to be demonstrated [3]. They would play an important role in the penetration of the spermatozoon into the cumulus oophorus [4] and in the processes involved in fertilization [5]
In order to investigate the in vitro effect of EOs on physiological parameters of human sperm and to choose the ideal exposure time of each HE with sperm, a test was performed on four semen samples classified as normal

Summary

Introduction

Infertility is one of the new plagues of the 21st century and more and more couples are affected each year. Male infertility is reflected in 61% of cases by quantitative (sperm count) and/or qualitative (decreased mobility, vitality and sperm morphology) abnormalities [2]. We were interested in qualitative anomalies including mobility and vitality. Because the importance of these two parameters on the chances of conceiving in vitro fertilization (IVF) or naturally is no longer to be demonstrated [3]. They would play an important role in the penetration of the spermatozoon into the cumulus oophorus [4] and in the processes involved in fertilization [5]

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