The effect of superposition of 900 MHz and incoherent noise electromagnetic fields on the induction of reactive oxygen species in SP2/0 cell line

Abstract

Background: Inducon of cellular response aer exposure to electromagnec fields is limited to coherent fields. An incoherent noise field is supposed to suppress the bioeffects of regular RF electromagnec fields. The purpose of this study was to invesgate the effect of GSM mobile phone-induced radiofrequency (RF) on the inducon of oxidave stress in SP2/0 cell line. Materials and Methods: This study was also an a-empt to assess whether these RF-induced effects can be blocked by superposing the RF radiaon and an incoherent magnec noise. Three groups of cultured cells were used in this study. The cells in the first group were only exposed to RF radiaon emi-ed from a mobile phone simulator. The second group was only exposed to an incoherent noise field and the third group was simultaneously exposed to RF radiaon and incoherent noise field. The exposure duraon in all groups was 2 hours. The level of ROS producon in the cells was quanfied by the CM- H2DCFDA fluorescence probe, using flow cytometry technique. Results: Although our results showed increased ROS producon aer exposure to 900 MHz RF radiaon, superposion of 900 MHz RF and the incoherent noise fields did not lead to increased levels of ROS in any experiment. However, the differences between RF exposure group and superposion of RF and noise exposure group were not stascally significant. Conclusion: Altogether our results cannot support the neutralizing effect of noise theory but may confirm the concept that just the coherent fields can be bioeffecve while the incoherent noise fields cannot cause any biological effects.