Abstract
The purpose of this study was to evaluate the responses of coronary and pulmonary arteries to the generation of superoxide (O2.) via organic redox complex formation between ascorbic acid and imidazole. Imidazole facilitates the transfer of electrons from ascorbic acid to O2 resulting in the generation of O2. Using a Clark style electrode, oxygen levels were monitored in solutions of ascorbic acid and imidazole and decreasing oxygen levels were used as an indication of O2. generation. In another series of experiments, small rings of porcine coronary and pulmonary arteries were dissected, mounted in isolated organ baths, and administered increasing concentrations (10−8–10−3 M) of ascorbic acid, glycine, imidazole, or ascorbic acid and imidazole. Vascular responses were recorded as changes in tension. The combination of ascorbic acid and imidazole, but not ascorbic acid or glycine, caused a significant vasoconstriction in both coronary and pulmonary arteries. Imidazole induced vasoconstriction in coronary arteries. Taken together, these results suggest that ascorbic acid in the presence of imidazole may increase O2. production resulting in an increase in tension in coronary and pulmonary arteries. This research was supported by a Grand Valley State University Research Grant‐in‐Aid.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.