The Effect of Supernatant Culture Medium Isolated from 3T3-L1 Cells Subjected to Low Power 980 and 650 nm Laser Radiation on Wound Healing Process

Abstract

Background: Fibroblasts are the most important cells in the healing process of wounds. The motility activity of low-level laser (light) therapy (LLLT) on fibroblast proliferation has been well-established in vitro. Laser treatment for scar removal increases the number of scars. Objectives: This method uses light therapy to remove the outer layer of the skin surface and produce new skin cells to cover the damaged skin cells. Methods: The present research is from an experimental laboratory. First, tissue fibroblast cells were cultured under appropriate conditions. Then, it was exposed to laser radiation with intensities of 650 and 980 nm, and its supernatant solution was used for wound treatment. Interleukin 2 (IL-2), tumor necrosis factor-α (TNF-α), and vascular endothelial growth factor (VEGF) levels were used to check the recovery. Results: The results of the MTT assay showed an increase in the viability of the cell line under laser irradiation. In addition, these evaluations showed an increase in IL-2, TNF-α, and VEGF after 650 and 980 nm laser irradiation compared to the control group after 48 hours. Conclusions: According to the present study, laser therapy has potential therapeutic potential for wound healing. However, more studies are suggested to increase the efficiency and speed up the treatment process.