Abstract
Summary Pretreatment of cabbage sections with nontoxic concentrations of the sulfhydryl reagents PCMB or IA decreased their resistance to freezing injury. Thiols had no effect. As the plants hardened, the SH reagents produced less and less of a decrease in their resistance to freezing injury. This result is explained by a decrease in or protection of protein SH. The decrease in freezing resistance is the opposite of what would be predicted by the SH hypothesis of freezing injury, if the SH reagents simply prevent intermolecular SS formation, but other evidence indicates that they also induce some protein unfolding. It is proposed that this unfolding favors the aggregation of proteins on freezing.
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