The Effect of Sucrose on Growth and Morphology of Rodent Tuber (Typhonium flagelliforme) Plantlets in Minimal Growth Preservation in vitro

Abstract

Rodent tuber (Thyphonium flagelliforme) is a herbal plant that has anticancer bioactive compounds. In vitro, conservation is attemptto preserve germplasm under aseptic conditions. Conservation techniques are used to store germplasm and minimize somaclonal variation. This study aims to identifysucrose’s effect on the minimum growth of rodent tuber in vitro culture and obtain the right sucrose concentration to store rodent tuber in vitro for extended shelf life. This study was used plantlets from Bogor and Pekalongan using ½AMS medium with 40, 50, 60, and 70 g/L sucrose treatment. In the treatment of 70 g/L sucrose concentration on rodent tuber from Bogor inhibited the increase of the number of shoots, number of leaves, root length, number of rootswas 0.50, 2.45, 1.43 cm, and 15.38, respectively. The growth of rodent tuber from Pekalongan on media with a sucrose concentration of 70 g/L resulted in the average number of shoots, number of leaves, root length, number of rootswas 0.98, 1.47,2.10 cm, and 13.74, respectively. Morphological changes in rodent tuber from Bogor and Pekalongan occurred in the number of shoots, the number of leaves, root length, and the number of roots with 70 g/L sucrose treatment.