Abstract
Hot ethanolic alkali as used in the saponification of fatty material has been shown to degrade PCDFs and PCDDs in proportion to their increasing chlorine content and, in the case of the PCDFs, to produce lower chlorinated PCDFs and ethoxy-PCDFs as artifacts. The reports in the earlier literature based on alkaline procedures of both the levels of PCDFs and the presence of non 2378-chlorine substituted PCDFs in human samples are erroneous reinforcing the conclusion that the persistent, toxic congeners in humans are those with 2378-chlorine substitution.
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