The effect of storage environment on the longevity of conidia of Beauveria bassiana

Abstract

The effect of air-dry storage environment on the longevity of conidia from seven isolates of Beauveria bassiana produced at different times and locations was determined by estimating the parameters of a viability equation. Conidia were stored hermetically at six to 11 moisture contents between 2.3 and 32.0% with one (50 ± 0.5 °C) to five constant temperatures (10, 20, 30, 40 and 50 ± 0.5°) for various periods up to 372 d and then tested for viability. All isolates behaved similarly (P > 0.25) in terms of the relative effect of moisture content (Cw) and temperature (CH and CQ) on conidial longevity; common values were Cw = 3.05 (SE = 0.07), CH = 0.0293 (SE = 0.0078), and CQ = 0.00081 (SE = 0.00011). Estimates of the low-moisture-content limit to the negative logarithmic relation between conidial moisture content and longevity were 4.6 and 5.0% at 50° and 40°, respectively, for isolate I98–1140ss, and 5.2 and 5.1% moisture content, respectively, for isolate I97–1111. Absolute longevity (KE) varied considerably (P < 0.005) among isolates, even within an isolate when conidia were produced at different locations. Among the eight samples of seven isolates, two cohorts were identified with respect to KE (P < 0.005): conidia of three isolates which were produced at Ascot had a common estimate of KE of 6.696 (SE = 0.170), whereas those produced at Nairobi or Carolina provided a lower estimate (6.203, SE = 0.029). This difference in KE means that for any given viability period in any given environment, the conidia produced in Ascot provided about three times the longevity of the other samples.