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Abstract
The effect of testosterone, dihydrotestosterone, 3 beta-androstanediol and oestradiol-17 beta on the morphology and RNA synthesis in human benign prostatic hyperplasia (BPH) in organ culture has been investigated. In hormone treated and untreated explants alike, the epithelium multiplied to form several layers. This effect was most marked after exposure to dihydrotestosterone. In explants grown in non-supplemented medium the epithelium showed some squamous changes; testosterone or dihydrotestosterone preserved the secretory character of the epithelium while oestradiol-17 beta caused cellular degeneration. The incorporation of 3H-uridine into RNA was studied by autoradiography. In the epithelium, testosterone or dihydrotestosterone raised the uptake significantly over that measured in the control explants, oestradiol-17 beta reduced it while 3 beta-androstanediol produced similar values to those found in the control explants. The incorporation of 3H-uridine in the smooth muscle cells was increased by testosterone and decreased by oestradiol-17 beta. A comparison with normal rat prostatic epithelium in organ culture showed that in the absence of androgens the incorporation of 3H-uridine was lower than in BPH and the effect of testosterone correspondingly greater. The results suggest that although the growth of human BPH in organ culture appears to be androgen dependent, it still remains hormone sensitive and can be influenced by steroid hormones in a similar manner to that in rat prostatic gland. They further show that the smooth muscle of the stroma is also hormone sensitive, a point which should be considered in the hormonal management of benign prostatic hyperplasia.
Highlights
I The incorporation of 3H-uridine into RNA was studied by autoradiography
The results suggest that the growth of human benign prostatic hyperplasia (BPH) in organ culture appears to be androgen dependent, it still remains hormone sensitive and can be influenced by steroid hormones in a similar manner to that in rat prostate gland
They further show that the smooth muscle of the stroma is hormone sensitive, a point which should be considered in the hormonal management of benign prostatic hyperplasia
Summary
The hyperplastic prostatic tissue was obtained by transurethral resection from 6 patients between 62 and 86 years of age. After 6 days' growth, one set of control and one set of hormone treated explants were fixed in Bouin's solution, dehydrated, embedded in paraffin, sectioned at 6 ,tm and stained with haematoxylin and eosin for histological observation. The uptake of 3H-uridine was assessed over the epithelium in explants grown in non-supplemented medium and explants exposed to testosterone, DHT, 3/ diol and oestradiol-173, over the cells of the smooth muscle in control explants or after exposure to testosterone, DHT and oestradiol-17/. In explants grown in the presence of testosterone, some alveoli were lined with one row of columnar cells similar to those in the in vivo controls; in others, epithelial cell proliferation was increased but the cells remained columnar, exuded studied in rat prostatic epithelium in secretory matter into the lumen and explants kept in non-supplemented medium showed no evidence of squamous transor after exposure to testosterone. Most alveoli were lined with 4-8 rows of columnar cells and many showed a was counted and expressed as the average combination of hyperplastic columnar number of grains per cell and its standard epithelium on one side of the alveolus deviation
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