The effect of starvation on the planarian Arthurdendyus triangulatus (Tricladida: Terricola) as measured by stable isotopes

Abstract

Many studies of the population dynamics of the planarian Arthurdendyus triangulatus have been confounded because it has not been possible to define if small flatworms are adults that have been starved or young ones being recruited into the population. Body shrinkage is a process exhibited by planarians under nutritional stress. In birds and the few invertebrate species studied, starvation leads to a 15N-enrichment of body tissues (i.e. an increased δ15N). Under laboratory conditions, the posterior parts of flatworms were removed at day 0 and frozen and compared isotopically with the anterior parts of flatworms, which were starved for 67, 108, 171 and 243 days. With one exception, δ13C 108 days after starvation, the impact of starvation on A. triangulatus %N, %C and δ13C was not significant statistically. In contrast, δ15N increased significantly (to a maximum enrichment of 2.8‰ by day 243) on all sampling dates. This is supported by significant positive correlations between the changes in δ15N between the anterior and posterior body parts and number of days after starvation (p≤0.05) and decrease in body weight (p≤0.01), respectively. Existing δ15N data from field populations of animals show intra-population variability of around 5.0‰, greater than that noted as a result of starvation. Thus, as a single measure, it is unlikely that δ15N can be used to accurately characterise the proportion of starved A. triangulatus within a field population.